After graduating from the Bruges art school, Ann Demeuleester studied fashion design at the Royal Academy of Fine Arts in Antwerp from 1978 to 1981. In 1981 she graduated with the class that became known as the "Antwerp Six", [ 1] or a group of stylists who have become particularly influential in the fashion field, which also includes Walter Van Beirendonck, Dries van Noten, Dirk Van Saene, Dirk Bikkembergs and Marina Yee.
Since 1981 the designer has worked as a freelance for several international fashion houses, until 1985, when she founded her own brand together with her husband, photographer Patrick Robyn.  Demeuleester presented its first women's collection in Paris in 1992 , alongside the men's collection in 1996.  The Ann Demeuleester brand becomes famous for its minimalist and androgynous style, which combines elements of men's fashion with those of women's wardrobes. 
Ann Demeulemeester opened her first boutique in Antwerp in 1999 and currently her collections, which in addition to clothing also include accessories and footwear, are sold in over 30 countries around the world. 
In November 2013, Ann Demeulemeester announced her farewell to the fashion house. The release was made known through a letter written by Ann herself and in which there was also written that the brand would present the women's and men's collection in autumn / winter 2014 at the women's fashion week in Paris.